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. 2015 Jul 7;12(4):5086–5092. doi: 10.3892/mmr.2015.4049

Figure 4.

Figure 4

Girdin regulates glioma cell migration and invasion via the PI3K-Akt signaling pathway. (A and B) Western blot analysis was performed to detect changes in the levels of p85α, p110α, p-Akt and Akt following transfection with girdin shRNA. (C and D) Western blot analysis was performed to detect the effects of girdin silencing on the protein levels of p-Akt, Akt and MMP-9 following treatment with LY294002. (E and F) A Transwell invasion assay was performed to detect the effect of girdin silencing on cell invasion following treatment with LY294002. Images were captured under a light microscope (magnification, ×200). Each experiment was repeated three times. The experimental results are presented as the mean ± standard deviation. **P<0.01, compared with the negative control. shRNA, short hairpin RNA; NC, negative control; DMSO, dimethyl sulfoxide; MMP, matrix metalloproteinase; Akt, protein kinase B; p-, phosphorylated.