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Analysis of apoptosis using flow cytometry in HepG2 cells. Cells were treated with various concentrations of oleanolic acid. (A–D) represent treatment with 0, 5, 25 and 50 µM oleanolic acid, respectively, for 48 h. Binding of Annexin V/fluorescein isothiocyanate to phosphatidyl serine was determined by flow cytometry. The percentage of apoptotic cells determined by the number of Annexin V/propidium iodide-positive cells is shown in the scattered plot.
