Figure 4.

GGA attenuates profibrogenic protein expression in CCl₄-induced rats. (A) Immunofluorescence was used to examine the expression levels of TGF-β1 and α-SMA in the liver tissues (magnification, ×200). (B) Protein samples (50 µg) were loaded for western blot analysis of TGF-β1 and α-SMA. Similar results were obtained from three repeated experiments. (C) Quantitative analysis was performed for TGF-β1 and α-SMA using a densitometer. Data are presented as the mean ± standard deviation. ^#P<0.05, compared with the control group; ^*P<0.05 compared with the CCl₄ group. GGA, geranylgeranylacetone; CCl₄, carbon tetrachloride; TGF-β1, transforming growth factor-β1, α-SMA, α-smooth muscle actin.