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. 2015 Jul 29;12(4):5737–5745. doi: 10.3892/mmr.2015.4146

Figure 1.

Figure 1

Example of the analysis of glycosylation at Asn10 by tandem MS. MS/MS spectrum of peptide INTITFDAGNATINK with HexNAc attached to Asn10. The two ion series result either from fragmentation of peptide-HexNAc or by initial loss of HexNAc. Accordingly, there are two series of fragment ions in the corresponding parts of the spectrum; those assigned to peptides with the initial loss of HexNAc are marked by apostrophes. Open circles indicate peaks corresponding to loss of water from the indicated fragment ion. P, parent ion. Numerical values for the two series of fragment ions are provided in the accompanying table, with those identified in the spectrum highlighted in boxes. HexNAc, N-acetylhexosamine; MS, mass spectrometry.