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. 2015 Jul 3;12(4):5100–5108. doi: 10.3892/mmr.2015.4039

Figure 3.

Figure 3

Akt is involved in BrMC-induced apoptosis. (A and B) Expression levels of t-Akt and p-Akt (Ser473) were detected by western blot analysis in A2780cells and A2780/DDP cells. Cells were treated with BrMC (2.5 µM) for 24 h, then lysed and assayed for individual protein levels by western blot. β-Actin was used as a control. p-Akt and Akt levels were measured by densitometric analysis of the western blots and compared to actin levels. (C) Detection of the role of LY294002 on apoptosis of cells. Cells were treated with BrMC and/or 20 µM LY294002 for 24 h, and then individual protein levels were detected by western blotting. (D) Apoptotic rate of the A2780 and A2780/DDP cells. All data are depicted graphically as the mean ± standard error of the mean for at least three independent experiments. **P<0.01; #P<0.05, vs. treatment with BrMC (5 µm) and LY294002 (20 µm). p/t-Akt, phosphorylated/total Akt; BrMC, 8-bromo-7-methoxychrysin; DDP, cisplatin.