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. 2015 Jul 13;12(4):4995–5002. doi: 10.3892/mmr.2015.4075

Figure 3.

Figure 3

Galectin-3 (gal-3) knockdown reduces the oxidized low-density lipoprotein (oxLDL)-induced activation of human umbilical smooth muscle cells (HUSMCs). (A) Following transfection with either control or gal-3-specific small interfering (si)RNA for 48 h, cellular migration was analyzed using a transwell assay. The cells that migrated from the upper to the lower chamber were counted in five non-overlapping fields under a microscope (magnification, ×100). The cells that migrated to the lower surface of each chamber are shown. (B) The cells transfected with scramble siRNA were defined as control and set to 100. Following transfection with either control or gal-3-siRNA for 24 h, HUSMCs were incubated for 48 h in the absence or presence of 50 μg/ml oxidized low-density lipopolyprotein (oxLDL). *P<0.05, vs. control; #P<0.05, vs. oxLDL. (C) Following transfection, the HUSMCs were incubated for 24 or 48 h in the absence or presence of 50 μg/ml oxLDL. A Cell Counting kit-8 assay was used to detect cell proliferation. The cells transfected with scramble siRNA were defined as control. (D) The HUSMCs were stained with Oil Red O in order to detect neutral lipids. Representative images (magnification, ×40) of the cells stained with Oil Red O are shown. Data are presented as the mean ± standard deviation. *P<0.05, vs. the control. #P<0.05, vs. oxLDL.