Figure 1.

Imaging modalities for MSC-tumor tropism. A) Flow cytometry plot of cells from a single cell suspension of a digested primary tumor containing three populations of cells (Tumor cells, MSCs, and residual mouse mammary cells.) B) eGFP fluorescent MSCs (green) and glioma tumor cells (red) identified using epifluorescence microscopy of tumor sections show co-localization within tumor. Bar = 100 μm. C) Whole-chromosome FISH painting identifies Y chromosomes (red) from male rat MSCs within female-derived glioma tumors. Nuclei are stained with by DAPI (4′,6-diamidino-2-phenylindole staining) (blue). Scale bar = 15 μm. D) Xenogen images demonstrate the use of BLI to track fLuc-expressing MSC homing from tail-vein specifically to primary orthotopic 4T1 breast tumors (below) compared to non-cancerous mammary fat pads (above). E) Intravenously-delivered SPIO-loaded MSCs localize to lung metastases and can be visualized by MRI. 1, Normal lung. 2,5) Lung with MDA-MB-231 metastases. 3,6) Same lung 1 hr after SPIO-loaded MSC injection shows decreased MRI signal in metastases signal. 4) H&E histologic sections (bar, 100 μm). See references for more details. Figure 1A (unpublished data from our lab). Figures 1B and 1C from Reference 23, Figure 1D and 1E adapted and reprinted by permission from the American Association for Cancer Research from references 18 and 14 respectively.