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. Author manuscript; available in PMC: 2015 Sep 24.
Published in final edited form as: Clin Immunol. 2011 Feb 26;139(3):302–313. doi: 10.1016/j.clim.2011.02.014

Figure 4.

Figure 4

Cytokine and degranulation profile of 15mer peptide short-term in vitro-expanded T-cells. A. Representative intracellular cytokine FACS plots showing CFSE dilution versus TNFα (upper) and TNFα vs IFNγ (lower) for four HCC patients with glypican-3-specific TNFα responses after 1 week of in vitro expansion using 15mer pooled peptides. B. Example in which PBMC from HLA-A2+ HCC patient were expanded with 9–10mer optimal peptides. Pentamer frequency ex vivo (top left) and after expansion in vitro (bottom left) for Sur1M2 and 5 GPC3 pentamers are shown. Ex vivo IFNγ Elispot (top right) shows no IFNγ+ responses against any glypican-3 peptide with lack of increase in IFNγ production by Elispot after restimulation with cognate antigen (bottom right).