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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Clin Immunol. 2015 May 16;160(2):261–276. doi: 10.1016/j.clim.2015.05.005

Figure 7. Methylation of CD19 promoter region in healthy control horses and equine CVID patients.

Figure 7

Methylation status was determined by amplification of bisulfite-treated bone marrow genomic DNA and sequencing of 10 unique clones per horse. Transcription factor binding sites are labeled above motif sequence and CpG sites are marked by black rectangles. Average methylation of each CpG is shown by circles shaded according to level of methylation. DNA from 6 healthy horses had 44.3% methylation in this region and CVID-affected horses (n=7) had 74.0% total methylation, a significant increase (p = 0.000).