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Oil Red O staining and quantification. A) ASCs cultured in adipogenic differentiation medium for 1, 3, 5, and 7 days were stained with Oil Red O. Scale bar corresponds to 400 μm. B) Quantification of staining demonstrated a significant increase in triglyceride and lipid accumulation at Days 5 and 7 (*p < 0.05, ***p < 0.001).
