Fig. 4.

Showing the expression of different epithelial and basement membrane markers among the 2 equivalents (bladder stroma cells [BSCs]-based and DF-based equivalents). Although both equivalents revealed expression of general epithelial markers (AE 1,3), uroplakin III (UPIII) and tight junctions E-cadherin, however they had different expression of cytokeratin 20 and 14. BSCs-based equivalents had terminally differentiated urothelium with evident expression of CK 20 and minimal expression of CK14, while DF-based equivalents showed the opposite. There was well-defined intact basement membrane as shown by positive staining for Laminin V and Collagen IV.