Number of infusions earned over 10 acquisition sessions of nicotine self-administration. A 3 × 2 × 10 ANOVA testing the effects of group, nicotine dose, and day revealed significant main effects of group (p < 0.05) and day (p < 0.05), and several significant interactions [group X day (p < 0.05), group X nicotine dose (p < 0.05), and day X group X nicotine dose interaction (p < 0.05)]. Follow-up 2 × 2 ANOVAs to further delineate the effect of group as described in section 2.4 were conducted on the last three sessions. These analyses failed to reveal a significant effect of adding cocktail to the nicotine solution; however, adding TCP to the cocktail solution significantly increased infusions earned (p < 0.05) in a manner that depended on nicotine dose (p < 0.05). Nicotine + cocktail + TCP also differed from nicotine alone (p < 0.05), although this was not significantly altered by nicotine dose (p > 0.05). Pairwise comparisons revealed no significant effects of dose. Significant pairwise comparison to nicotine + cocktail is indicated by Φ, p < 0.05, and significant pairwise comparison to nicotine only indicated by #, p < 0.05. Error bars represent standard error.