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. Author manuscript; available in PMC: 2016 Oct 5.
Published in final edited form as: Vaccine. 2015 Aug 28;33(41):5386–5395. doi: 10.1016/j.vaccine.2015.08.062

Figure 2. VLPV-PSA vaccine induces dose-dependent PSA-specific immune response in DR2bxPSA F1 mice.

Figure 2

Mice were immunized i.m. twice with VLPV-PSA at indicated doses. Cellular immune responses to PSA were measured in the spleens (A) and inguinal LN (B) two weeks after boost immunization using IFNγ ELISPOT assay. Lymphocytes (2×105 per well) were stimulated with either H-2Db restricted peptide PSA65–73 or TRAMP-PSA tumor targets. Irrelevant peptide Neo49–59, parental TRAMP-C1 tumor cells or media (No Ag) were used as negative controls. Target tumor cells were pre-treated with IFNγ and irradiated at 10,000 rad. The data for the pool of 3 mice per group are shown (mean ± SD of triplicates). TNTC: too numerous to count.