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. Author manuscript; available in PMC: 2016 Nov 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 Aug 1;415:12–23. doi: 10.1016/j.mce.2015.07.029

Figure 3.

Figure 3

Effect of wortmannin and LY294002 (1 h) on ERK1/2 activation by GnRH and PMA in αT3-1 cells. αT3-1 cells were serum-starved for 16 h before treatment with wortmannin (wort), 10nM or 10μM, or LY294002 (LY), 10μM or 100μM, for 1 h before stimulation with GnRH or PMA (100nM each) for 5 min. After treatment cell lysates were analyzed for ERK1/2 activity by Western blotting using an antibody for phospho-ERK1/2 (pERK1/2). Total ERK1/2 (gERK1/2) was detected with polyclonal antibody as a control for sample loading. A representative blot is shown and similar results were observed in five other experiments. The results are shown as mean±S.E.M of maximal phosphorylation. **p-val≤0.01; *p-val≤0.05 vs. GnRH or PMA alone.