Skip to main content
. Author manuscript; available in PMC: 2016 Nov 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 Aug 1;415:12–23. doi: 10.1016/j.mce.2015.07.029

Figure 7.

Figure 7

Time course of AKT activity by GnRH, PMA and IGF-1 in αT3-1 cells. αT3-1 cells were serum-starved for 16 h, followed by stimulation with GnRH, PMA (100nM each) or IGF-1 (20ng/ml) for up to 240 min. After treatment cell lysates were analyzed for AKT activity by Western blotting using an antibody for phospho-AKT- Ser473 (pAKTser473) or phospho-AKT-Thr308 (pAKTthr308). Total AKT was detected with polyclonal antibody as a control for sample loading. A representative blot is shown and similar results were observed in three other experiments. The results from three experiments are shown as mean±S.E.M of control phosphorylation.**p-val≤0.01; *p-val≤0.05 vs. control.