Table 3.

Kinetic Parameters of a Single dNTP Incorporation at 37 °C

dNTP	Kd,dNTP (µM)	kp (s−1)	kp/Kd,dNTP (µM−1 s−1)	efficiency ratioa	fidelityb	probabilityc
19/26-mer-dG1,8
dGTP	288 ± 63	2.3 ± 0.2	8.0 × 10−3	3.1		99.7
dATP	571 ± 103	(7.2 ± 0.6) × 10−3	1.3 × 10−5	1.1	1.6 × 10−3	0.2
dCTP	511 ± 148	(1.4 ± 0.2) × 10−3	2.7 × 10−6	51.9	3.4 × 10−4	0.03
dTTP	1799 ± 242	(1.08 ± 0.09) × 10−2	6.0 × 10−6	1.5	7.5 × 10−4	0.07
20/26-mer-dG1,8
dCTP	219 ± 17	(1.05 ± 0.03) × 10−2	4.8 × 10−5	1188		93.4
dATP	1149 ± 220	(1.1 ± 0.1) × 10−3	9.6 × 10−7	19.8	2.0 × 10−2	1.9
dGTP	424 ± 90	(1.7 ± 0.1) × 10−4	4.0 × 10−7	112.5	8.3 × 10−3	0.8
dTTP	130 ± 56	(2.6 ± 0.3) × 10−4	2.0 × 10−6	41.5	4.0 × 10−2	3.9
21/26-mer-dG1,8
dGTP	681 ± 130	(1.9 ± 0.2) × 10−1	2.8 × 10−4	13.2		97.9
dATP	1630 ± 253	(1.0 ± 0.1) × 10−3	6.1 × 10−7	3.9	2.2 × 10−3	0.2
dCTP	2007 ± 207	(8.6 ± 0.6) × 10−3	4.3 × 10−6	7.2	1.5 × 10−2	1.5
dTTP	937 ± 36	(9.8 ± 0.2) × 10−4	1.0 × 10−6	1.8	3.6 × 10−3	0.4
22/26-mer-dG1,8
dCTP	458 ± 87	2.3 ± 0.2	5.0 × 10−3	6.8		99.5
dATP	1009 ± 317	(4.2 ± 0.7) × 10−3	4.2 × 10−6	1.0	8.4 × 10−4	0.1
dGTP	273 ± 20	(3.3 ± 0.1) × 10−3	1.2 × 10−5	0.71	2.4 × 10−3	0.2
dTTP	1352 ± 181	(1.3 ± 0.1) × 10−2	9.6 × 10−6	1.1	1.9 × 10−3	0.2

^aCalculated as (k_p/K_d,dNTP)_undamaged /(k_p/K_d,dNTP)_damaged. Values for (k_p/K_d,dNTP)_undamaged are from ref 41. “Undamaged” indicates undamaged DNA, while “damaged” denotes DNA containing a template of 26-mer-dG^1,8.

^bCalculated as (k_p/K_{d,incorrect dNTP})_damaged/[(k_p/K_{d,correct dNTP})_damaged + (k_p/ K_{d,incorrect dNTP})_damaged].

^cCalculated as ((k_p/K_d,dNTP)_damaged /[Σ(k_p/K_d,dNTP)_damaged]) × 100.