. Author manuscript; available in PMC: 2016 Sep 23.

Published in final edited form as: J Am Chem Soc. 2015 Sep 11;137(37):12131–12142. doi: 10.1021/jacs.5b08027

Table 4.

Biphasic Kinetic Parameters for Correct dNTP Incorporation onto 5′-[³²P]-Labeled DNA (30 nM) in the Presence of an Unlabeled DNA Trap (5 µM) at 37 °C

DNA substrate	correct dNTP	A_f (nM)^a	k_f (s⁻¹)	A_s (nM)^a	k_s (s⁻¹)
19/26-mer	1.2 mM dGTP	26.7 ± 0.7 (89%)	1.8 ± 0.2
20/26-mer	1.2 mM dCTP	26.0 ± 0.6 (87%)	2.8 ± 0.3
21/26-mer	1.2 mM dGTP	25.2 ± 0.6 (84%)	0.49 ± 0.04
19/26-mer-dG^1,8	1.2 mM dGTP	22.9 ± 0.6 (76%)	0.74 ± 0.06
20/26-mer-dG^1,8	0.2 mM dCTP	5.4 ± 0.5 (18%)	0.014 ± 0.003	18.7 ± 0.9 (62%)	(2.4 ± 0.4) × 10⁻⁴
	0.7 mM dCTP	5.6 ± 0.4 (19%)	0.039 ± 0.007	18.1 ± 0.6 (60%)	(4.6 ± 0.4) × 10⁻⁴
	1.2 mM dCTP	5.4 ± 0.5 (18%)	0.043 ± 0.007	18.2 ± 0.5 (61%)	(1.9 ± 0.1) × 10⁻³
21/26-mer-dG^1,8	1.2 mM dGTP	24.4 ± 0.4 (81%)	0.075 ± 0.005

Calculated as (reaction amplitude/30 nM) × 100.