Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2016 Nov 1.

Published in final edited form as: Biochim Biophys Acta. 2015 Aug 29;1852(11):2525–2534. doi: 10.1016/j.bbadis.2015.08.026

Freshly isolated and stored platelets (10×10⁶/well) were plated on Cell-Tak coated plates and mitochondrial stress test was performed by establishing basal OCR followed by sequential injection of 1 µg/ml oligomycin, 0.6 µM FCCP and 10 µM antimycin A. (A) Representative OCR traces of mitochondrial stress test. Indices of mitochondrial function – (B) Basal, (C) ATP-linked (AL), (D) Proton leak (Pl), (E) Maximal (Max), (F) Reserve capacity (RC), (G) Non-mitochondrial (NM) OCR and (H) BHI [(ALxRC)/(PLxNM)] were calculated. The indices are represented in a box plot with lower 25^th percentile, median, upper 75^th percentile, and whiskers drawn at 1.5 × interquartile range. n = 35 individual donors for the freshly isolated and n = 38 individual bags for the stored. *p<0.05, **p <0.01, different from freshly isolated.

Freshly isolated and stored platelets (10×10⁶/well) were plated on Cell-Tak coated plates and mitochondrial stress test was performed by establishing basal OCR followed by sequential injection of 1 µg/ml oligomycin, 0.6 µM FCCP and 10 µM antimycin A. (A) Representative OCR traces of mitochondrial stress test. Indices of mitochondrial function – (B) Basal, (C) ATP-linked (AL), (D) Proton leak (Pl), (E) Maximal (Max), (F) Reserve capacity (RC), (G) Non-mitochondrial (NM) OCR and (H) BHI [(ALxRC)/(PLxNM)] were calculated. The indices are represented in a box plot with lower 25^th percentile, median, upper 75^th percentile, and whiskers drawn at 1.5 × interquartile range. n = 35 individual donors for the freshly isolated and n = 38 individual bags for the stored. *p<0.05, **p <0.01, different from freshly isolated.