Fig. 4.

Classification tree based on neutrophil chemokines CXCL1, CXCL2, CXCL5. Female 8-week-old non-sibling DO mice and C57BL/6J mice were infected with ∼100 M. tuberculosis bacilli by aerosol. From the first experiment, complete data for all 15 molecular parameters used for model generation and training were obtained from 70 M.-tuberculosis-infected DO mice and eight non-infected DO mice. The best-performing classification tree used only lung and plasma CXCL1, CXCL2, CXCL5 (A), which was then validated by the leave-one-out method (B). After validation, performance of the classification tree was stringently tested using data from the second independent experiment, which included 52 M.-tuberculosis-infected DO mice, three non-infected DO mice and five M.-tuberculosis-infected C57BL/6J mice (C), with complete data from all 15 molecular parameters.