Fig. 5.

In E6 chick embryos, hADSC migrated associating with peripheral nerves and also formed blood vessels. In situ hybridization with Alu probes, immunostaining with HNK1 antibody and Alcian blue counterstaining in cross-sections at the anterior limb bud level. (A,A′) When hADSC were grafted on the level of the aortic plexus (a.p.), human cells were found ventrally until the dorsal aorta (d.a.), associated with HNK1+. A few cells presented a HNK1+ cytoplasm (A″, green arrows), although most of them were HNK1− (A‴). (B) hADSC were located in the d.a. wall. Note other cells lateral to the neural tube (n.t.) and in the ventralmost region of the d.a. (black arrows). (B′,B″,B‴) Human cells were perivascular, while the endothelium was chicken-derived (red arrows). (C,C′) hADSC were found in the mesonephros (m.). (C″) Human cells were in contact with the basal lamina of the Wolffian channels (w.c.), in a perivascular location. Again, the endothelium was derived from chicken. A,B,C, scale bar: 200 µm; A′,B′,C′, scale bar: 50 µm; A″,A‴,B″,B‴,C″, scale bar: 10 µm. c., coelom; gl., glomerulus; v., blood vessel.