Fig. 2.

Yap8 interacts with Ufd2 upon arsenic stress. (A) Yeast two-hybrid assays reveal Ufd2 as a Yap8-interaction partner. The Y187 strain was co-transformed with plasmids encoding GAL4^DBDLam/GAL4^ADT, GAL4^DBDp53/GAL4^ADT, GAL4^DBD/GAL4^ADUFD2, GAL4^DBDYAP8/GAL4^ADor GAL4^DBDYAP8/GAL4^ADUFD2 and β-galactosidase activity was measured in cells challenged or not with 2 mM As(V) (MU, Miller Units). Values represent the mean±standard deviation (s.d.) of three biological replicates and statistical differences denoted as *P<0.05. (B) Ufd2 co-immunoprecipitates together with Yap8. Y187 cells co-transformed with GAL4^DBD/GAL4^ADUFD2 (lanes 1 and 4), GAL4^DBDYAP8/GAL4^ADYAP8 (lanes 2 and 5) or GAL4^DBDYAP8/GAL4^ADUFD2 (lanes 3 and 6) were exposed to 2 mM As(V) for 60 min and Gal4^DBDYap8, bearing a c-myc epitope, was immunoprecipitated with anti-c-myc antibody. Immunoblotting was performed using anti-HA, anti-c-myc and anti-Pgk1 antibodies. A representative experiment is shown. IP, immunoprecipitation; IB, immunoblotting.