Table 1.
Summary of FOXO1 localization in primary cortical neuronsa
| FOXO1 | 249A | 249D | AAA | AAA-249A | AAA-249D | |
|---|---|---|---|---|---|---|
| Cdk5+/+ | C | C | C | N | N | C + N |
| Cdk5−/− | C + N | C + N | C | N | N | C + N |
| Roscovitine | C + N | C | C | N | N | C + N |
| Cdk5/p35 or Cdk5/p25 | C | C | C | C + N | N | C + N |
| Cdk5-KD/p25 or Cdk5-KD/p35 | C | C | C | N | N | C + N |
| LY294002 | N | N | C + N | N | N | C + N |
| Cdk5/p35 or Cdk5/p25 + LY294002 | C + N | N | C + N | |||
| Cdk5-KD/p35 or Cdk5-KD/p25 + LY294002 | N |
aThe indicated plasmids were transfected into Cdk5+/+ or Cdk5−/− neurons on DIV 3; drug treatment was administered if desired on DIV 5. The localizations of FOXO1 were detected on DIV 7.