Table 1.
Binding data (indicated by 1/Ki(uM)*1000) of the DhelOBP21 and its mutant with different ligands.
| Ligand | WT | S67A | I84N | T119N | ||||
|---|---|---|---|---|---|---|---|---|
| pH7.4 | pH5.0 | pH7.4 | pH5.0 | pH7.4 | pH5.0 | pH7.4 | pH5.0 | |
| S-(-)-Limomeme | 43.80 | 7.95 | 21.26 | 60.57 | 33.99 | 43.73 | 42.54 | 45.88 |
| Terpinolene | 30.54 | 24.93 | 19.58 | 52.63 | 46.78 | 17.96 | 23.95 | 45.92 |
| (+)-α-Pinene | 53.73 | 29.52 | 34.44 | 60.36 | 59.36 | 47.90 | 28.03 | 28.73 |
| 3-Canene | 42.10 | 19.26 | 16.04 | 43.37 | 14.99 | 21.33 | 5.99 | 40.60 |
| (+)-β-Pinene | 49.12 | 36.80 | 29.17 | 64.70 | 26.40 | 26.79 | 38.73 | 14.94 |
| Myrcene | 70.37 | 45.37 | 25.69 | 82.00 | 22.26 | 24.72 | 34.05 | 25.22 |
| Camphene | 49.02 | 37.72 | 4.65 | 82.51 | 4.45 | 32.92 | 9.10 | 35.95 |
| β-Caryophyllene | 6.91 | 6.59 | 7.16 | 0.00 | 0.00 | 0.00 | 0.93 | 0.00 |
| (+)-α-Longipinene | 0.42 | 21.27 | 6.65 | 5.37 | 1.04 | 0.00 | 0.08 | 20.29 |
| (-)-Isolongifolene | 2.72 | 0.68 | 0.51 | 0.89 | 0.00 | 0.00 | 0.00 | 0.00 |
| (+)-Sativene | 16.60 | 1.93 | 1.64 | 14.18 | 2.03 | 0.05 | 6.11 | 4.66 |
| (+)-Longifolene | 0.14 | 2.31 | 2.09 | 0.00 | 0.35 | 0.00 | 0.24 | 3.27 |
| (-)-Caryophyllene oxide | 20.80 | 63.25 | 37.77 | 88.38 | 19.63 | 45.89 | 26.45 | 27.52 |
| Butylated hydroxytoluene | 29.55 | 22.69 | 14.80 | 88.15 | 22.74 | 50.42 | 57.06 | 42.67 |
| Camphor | 49.11 | 38.94 | 20.11 | 79.19 | 29.95 | 38.28 | 24.82 | 37.04 |
| 2-Methoxy-4-vinylphenol.Kosher | 31.18 | 16.99 | 13.97 | 64.47 | 10.53 | 28.32 | 29.01 | 22.05 |
| (-)-Fenchone | 49.72 | 36.93 | 39.04 | 128.47 | 9.00 | 35.96 | 28.86 | 46.58 |