Fig. 3.

Expression of miRNAs in hESC-derived neural progenitors. (A) The different groups of cells were generated by differentiating SOX1-GFP hESCs towards FB, MB or HB for 14 days. At that point, cells were sorted for GFP⁺ or CORIN⁺, generating the NE and FP groups, respectively. (B) Heat map showing a random clustering analysis in which each neural subtype clustered together, revealing that cells possess a specific global miRNA expression pattern. (C-M) miRNAs with a fourfold or higher expression in NE cells compared with hESCs were extracted, and the normalized counts for selected miRNAs are shown.