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. 2015 Sep 15;142(18):3178–3187. doi: 10.1242/dev.123851

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Generation of cortical neurons and astrocytes from pluripotent stem cells. (A) Cortical cultures were generated from hPSCs by SMAD inhibition combined with retinoid signalling. (B) Confocal image of day-25 forebrain cortical progenitors expressing OTX1/2, Nestin and phosphohistone H3 (PHH3). (C) Confocal image of day-70 cortical cultures containing GFAP-expressing astrocytes and Tuj1⁺ neurons. (D) Two-photon confocal image of day-72 cortical neurons showing 3D cultures expressing the deep layer cortical transcription factor Tbr1.