Fig. 1.

Domain organisation of Rpn13 and SGTA. (A,B) The discrete domains present in Rpn13 (A) and SGTA (B) are indicated together with the fragments of Rpn13 used for yeast two-hybrid analysis. For A: Pru, pleckstrin-like receptor for ubiquitin, C, C-terminal region of Rpn13 that binds UCH37. For B: N, N-terminal region responsible for both homo-oligomerisation and the binding of ubiquitin-like domains (UBLs); TPR, tetratricopeptide-repeat-containing domain; C, C-terminal glutamine-rich region implicated in binding to hydrophobic substrates.