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MiR-199a-5p regulates free cholesterol intracellular localization. Representative immunofluorescence analysis of free cholesterol (Filipin), F-actin and DiI–LDL in Huh7 cells transfected with non-targeting control miRNA (CM), miR-199a-5p mimic or inh-199a-5p and incubated with 30 μg/ml DiI–LDL for 1 h at 37°C. Fluorescence intensity plots are shown in the right panels. PM, plasma membrane. White arrows indicate the region of the cell subjected to image analysis. Scale bars: 10 μm.
