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. 2015 Sep 1;128(17):3250–3262. doi: 10.1242/jcs.169573

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Involvement of CD13 in 14-3-3ε-induced MMP-3 and MMP-13 release by murine articular chondrocytes. Mouse articular chondrocytes were transfected for 48 h with small interfering (siRNA) targeting the CD13 gene or with negative control AllStars. The cells were then starved and stimulated with recombinant 14-3-3ε (1 µg/ml) for 24 h. (A,C) Total RNA was extracted and mRNA levels of MMP-3 (A) and MMP-13 (C) were determined by qRT-PCR. (B,D) Protein levels of MMP-3 (B) and MMP-13 (D) in cell supernatants were measured by ELISA. 14-3-3ε-stimulated cells released 756.68±69 ng/ml and 347.3±48 pg/ml of MMP-3 and MMP-13, respectively. Bars show the mean±s.d. fold decrease in expression compared to the negative control-transfected chondrocytes stimulated with 14-3-3ε (set at 1) from three independent experiments. Blots in D are representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001; ns, not significant.