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. 2015 Sep 1;128(17):3210–3222. doi: 10.1242/jcs.169789

Fig. 1.

Fig. 1.

C-terminal deletion of LPPR1 abolishes its plasma membrane localization. (A,B) Representative images of Neuro2A cells expressing EGFP–LPPR1 (A) or LPPR1–HA (B), showing a similar distribution pattern. (C,D) Representative images of Neuro2A cells expressing EGFP–LPPR1 (C) or EGFP–LPPR1ΔC43 (D) together with a plasma-membrane-targeted mCherry (mem–Cherry) for visualization of overall cell morphology. The inset in C is a higher magnification image of the boxed area, showing the discontinuous and punctate distribution pattern of EGFP–LPPR1 along membrane protrusions (arrows). The inset in D is a higher magnification image of the boxed area, showing that EGFP–LPPR1ΔC43 was absent from membrane protrusions (arrows). (E) Quantification of the relative amount of intracellular and cell surface LPPR1. Quantitative results are mean±s.e.m. ***P<0.001 (two-way ANOVA and Bonferroni's multiple comparisons test). (F,G) Representative images of Neuro2A cells expressing EGFP (F) or EGFP–LPPR1CT (G). (H,I) Representative images and quantification of the protrusion numbers in cells overexpressing EGFP, EGFP–LPPR1, EGFP–LPPR1ΔC43 or EGFP–LPPR1CT. Quantitative results are mean±s.e.m. #P<0.05, ***P<0.001 (one-way ANOVA and Tukey's multiple comparisons test).