Skip to main content
. 2015 Sep 1;128(17):3210–3222. doi: 10.1242/jcs.169789

Fig. 4.

Fig. 4.

Co-transfection two of the LPPR members, except for co-transfection of LPPR3 and LPPR4, increases their protein levels. (A) Western blot and quantification (mean±s.e.m., n=4) showing increase in the EGFP–LPPR1 protein amount when co-transfecting EGFP–LPPR1 with either Flag–LPPR3, Flag–LPPR4 or Flag–LPPR5, compared to co-transfection with Flag control. (B) Western blot showing increase in the protein amount of Flag–LPPR3, Flag–LPPR4 or Flag–LPPR5 when co-transfected with EGFP–LPPR1, compared to co-transfection with EGFP control. (C) Western blot showing increase in the Flag–LPPR3 or the Flag–LPPR4 protein amount upon co-transfection of EGFP–LPPR5 with Flag–LPPR3 or with Flag–LPPR4, coinciding with an increase in EGFP–LPPR5. (D) Western blot showing no change in their protein level upon co-transfection of LPPR3 with LPPR4, compared to transfection with LPPR3 alone or LPPR4 alone. (E) Western blot showing that co-expression of EGFP–LPPR1 with Flag–LPPR3, Flag–LPPR4 or Flag–LPPR5 increased the phosphorylation of S6 (phospho-S6) ribosomal protein.