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. 2015 Sep 1;128(17):3263–3276. doi: 10.1242/jcs.171165

Fig. 1.

Fig. 1.

STX13 knockdown affects the melanocyte pigmentation and cargo transport. (A,B,E–G), Bright-field (BF) microscopy and immunofluorescence microscopy images of STX13-depeleted and control melanocytes. Arrows indicate the loss in pigmentation (A) or STX13 staining (B), and arrowheads (E–G) point to the localization of melanosome cargo to lysosomes in knockdown cells. Nuclei are stained with Hoechst 33258. The insets are a magnified view of the white boxed areas. Scale bars, 10 μm. (C,D,H,I) Visual quantification of melanocyte pigmentation (n=3, ≥100 cells/sample, ***P<0.001, mean±s.e.m.) (C), semiquantitative PCR (top) and immunoblotting analysis (bottom) (D), degree of colocalization between TYRP1 and LAMP-2, ***P<0.001, mean±s.e.m. (H), and immunoblotting analysis of melanosomal proteins (I) in STX13-knockdown cells.