Table 1. Comparison of Electron Transport, ATP Synthesis, and Menaquinone Content of M. smegmatisΔMSMEG1132 with those of M. smegmatis mc2 155a.
traits | M. smegmatis (WT) | M. smegmatis ΔMSMEG1132 | fold change |
---|---|---|---|
rate of electron transport (O2 equiv) | 0.12 ± 0.01 μmol of O2/h/ng of menaquinone | 0.04 ± 0.01 μmol of O2/h/ng of menaquinone | 3-fold reduction |
total cellular ATP | 6.4 ± 0.01 nmol/108 CFU | 6.2 ± 0.08 nmol/108 CFU | no change |
rate of ATP biosynthesis | 1.3 ± 0.01 nmol/min/108 CFU | 1.4 ± 0.01 nmol/min/108 CFU | no change |
total menaquinone (MK) | 4.3 ± 0.06 ng/108 CFU | 9.0 ± 1.6 ng/108 CFU | 2-fold increase |
Results are means of replicate observations ± standard deviation.