Fig. 5.

Cell and nuclear morphology under DMSO and nocodazole treatment. (A) Immunofluorescence images of untreated (this is same example as shown in Fig. 1A), DMSO control and nocodazole-treated MEF cells. Solid and open arrowheads indicate organized and disrupted microtubule networks, respectively. Note that the organization of actin filaments (green) remained intact whereas the α-tubulin-stained microtubule structure (red) was largely disrupted after a given nocodazole treatment (1 μM, 30 min). (B) Images of the nucleus in a DMSO-treated control MEF cell. Upper panel, z-depth rendering of the nucleus; lower panel, xz and xy cross-sectional views. (C) Images of the nucleus in a nocodazole-treated (1 μM, 30 min) MEF cell. Left panel, z-depth rendering of the nucleus; right panel, xz and xy cross-sectional views. Scale bars: 5 μm.