Table 3. Immunohistochemical gradient of P53 and P21 CIP1/ WAF1 in OSCC, para-tumor tissue and normal mucosa.
| Gradation of staining | |||||||
|---|---|---|---|---|---|---|---|
| Groups | Number | - | + | ++ | +++ | Positive | Positive rate/% |
| P53 | |||||||
| T | 50 | 16 | 5 | 16 | 13 | 34 | 68a |
| P1 | 50 | 20 | 10 | 12 | 8 | 30 | 60a |
| P2 | 50 | 27 | 12 | 7 | 4 | 23 | 46a |
| P3 | 50 | 41 | 5 | 4 | 0 | 9 | 18a |
| P4 | 50 | 50 | 0 | 0 | 0 | 0 | 0a |
| N | 20 | 20 | 0 | 0 | 0 | 0 | 0a |
| P21 CIP1/ WAF1 | |||||||
| T | 50 | 32 | 13 | 5 | 0 | 18 | 36b |
| P1 | 50 | 30 | 15 | 5 | 0 | 20 | 40b |
| P2 | 50 | 26 | 17 | 7 | 0 | 24 | 48b |
| P3 | 50 | 17 | 15 | 18 | 0 | 33 | 66b |
| P4 | 50 | 2 | 16 | 30 | 2 | 48 | 96b |
| N | 20 | 0 | 8 | 10 | 2 | 20 | 100b |
| eIF4E | |||||||
| T | 50 | 0 | 0 | 29 | 21 | 50 | 100c |
| P1 | 50 | 14 | 5 | 18 | 13 | 36 | 72c |
| P2 | 50 | 26 | 8 | 12 | 4 | 24 | 48c |
| P3 | 50 | 31 | 11 | 8 | 0 | 19 | 38c |
| P4 | 50 | 35 | 12 | 3 | 0 | 15 | 30c |
| N | 20 | 20 | 0 | 0 | 0 | 0 | 0c |
OSCC, oral squamous cell carcinoma. T, centre of the tumor; P1, 0–0.5 cm to tumor boundary; P2, 0.5–1 cm to tumor boundary; P3, 1–1.5 cm to tumor boundary; P4, 1.5–2 cm to tumor boundary; N, normal mucosa.
a
Significant differences of p53 expression were observed between T, P1, P2 and P3, P4 groups (P<0.05). No statistical difference of p53 expression was found between T and P1, P2 groups (P>0.05) and between P4 and N groups (P>0.05).
b
No significant differences between T, P1 and P2 groups (P>0.05), or between P4 and N groups (P>0.05); statistical differences between P2 and P3 groups (P<0.001), and between P3 and P4 groups (P<0.001).
c
Significant differences of eIF4E expression were observed between T, P1, P2, P3, P4 and N groups (P<0.005).