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. 2015 Sep 25;3:59. doi: 10.1186/s40478-015-0238-7

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© Atkinson et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Expression of C9ORF72 isoforms over development. a Relative expression of C9orf72 isoforms 1, 2 and 3 mRNA in combined cerebral cortex and hippocampus of C57Bl/6 mice. Isoform 1 was significantly (p < 0.05) higher at P1 compared to P7 and P56 and isoform 2 was significantly (p < 0.05) higher at P56 compared to P1 and P7. b Western blot of C9ORF72 expression in mouse tissue over development. Bands corresponding to reported isoforms of C9ORF72 were present at 55, 50 and 35 kDa (b). Additional bands at 110 and 50 kDa were also present. GAPDH was used as a loading control. Values represent mean ± standard error. *p < 0.05 P1 and P7 vs. P56