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. 2015 Sep 25;3:59. doi: 10.1186/s40478-015-0238-7

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© Atkinson et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression of C9ORF72 in synaptosome preparations from mouse brains. Figure shows representative western blots with results from 2 animals for each marker (indicated by 1 and 2 on figure). The 55 kDa isoform of C9ORF72 was present in the combined F3/F4 fractions which contain synaptosomes. C9ORF72 expression was low in fraction F1, containing membranes, and F2 containing myelin, membranes and glia. Unfractionated brain at P56 was also included. Purity of fractions was determined by labeling with GFAP (F2) and synaptic markers, synaptophysin, PSD-95 and GAD67 (F3/F4)