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. Author manuscript; available in PMC: 2015 Sep 25.
Published in final edited form as: Microbiol Immunol. 2015 Aug;59(8):452–465. doi: 10.1111/1348-0421.12282

Figure 5. IL-12 p40 and IL-10 regulation in BMDMs by NC101(pGEN-MCST5chuS) is mediated by a soluble factor secreted by live bacteria.

Figure 5

(A) Culture of BMDMs with WT, chuS overexpressing (pGEN-MCST5chuS), and chuS deleted(ΔchuS) E. coli NC101 in two chambers separated by a membrane permeable to substances <0.4um, resulted in decreased IL-12 p40 production and (B) increased IL-10 production by BMDMs incubated with the NC101(pGEN-MCST5chuS) compared to the other strains. Results are presented as mean±SEM from 3-4 independent experiments.