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. Author manuscript; available in PMC: 2016 Aug 19.
Published in final edited form as: FEBS Lett. 2015 Jul 17;589(18):2388–2393. doi: 10.1016/j.febslet.2015.07.010

Figure 2. S100P activates ERK and NF-kB signaling.

Figure 2

A. Serum-starved SW480 cells were induced with hr-S100P. Analysis of protein levels by western blots shows activation of ERK1/2 after 30min of cells with S100P. AP-1 components c-fos and c-jun are activated after 2hrs and 15min of hr-S100P treatment, respectively. NF-kB (p65) activation was on and off at 15min and 2hrs, respectively. Hr-S100P treatment increased expression of total c-fos (T-c-fos) at 15min and total p-65 at 1hr. Laminin B is the sample loading control. B. qRT-PCR analysis of SW480 cells induced with hr-S100P and treated with U0126 and CAY10512 inhibit ERK1/2 and NF-kB respectively, showing about 50% decrease in miR-21 expression levels. Error bars represent mean ± SEM, statistical significance was calculated using two tailed Student’s t-test, p< 0.05 =*, p< 0.01 = **, p< 0.001 = ***.