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. Author manuscript; available in PMC: 2016 Aug 19.
Published in final edited form as: FEBS Lett. 2015 Jul 17;589(18):2388–2393. doi: 10.1016/j.febslet.2015.07.010

Figure 3. S100P/RAGE signaling activates binding of AP-1 to the pri-miR-21 promoter.

Figure 3

A. pri-miR-21 promoter (1 to −1000 bp) within the sequence of chromosome 17 showing the AP-1 and NF-kB binding sites and regions within the primer sets (AP-1(1), AP-1(2) and NF-kB(2). NF-kB (1) primer set for NF-kB binding site is within the sequence of the pri-miR-21 precursor (AY699265). B. pri-miR-21 promoter activity decreases significantly in SW480 cells transfected with pri-miR-21 promoter constructs mutated on AP-1 binding sites (AP-1/I, AP-1/II, AP-1/III and AP-1/IV) and on NF-kB binding site 24hrs after hr-S100P treatment compared to WT promoter. C. c-fos binding to the pri-miR-21 promoter within the regions of AP-1(1) and AP-1(2) primer sets increases 3.5-fold enrichment compared to the control after 2hrs of hr-S100P treatment. Blue crosses represent mutation sites. Firefly luciferase activity was normalized to Renilla activity of the pri-miR-21 promoter constructs. Error bars represent mean±SEM, Statistical significance was calculated using two tailed Student’s t-test, p< 0.05 =*, p< 0.01 = **.