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. 2015 Sep 25;46:112. doi: 10.1186/s13567-015-0246-4

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© Corripio-Miyar et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Size and granularity of bovine myeloid sub-populations. Live single PBMC were gated as shown in Figure 2C and each highlighted in different colours for identification in side and forward scatter plots. The size and granularity of the populations was determined by their characteristic side and forward scatter plots: in red, CD14⁻CD16⁺⁺ (A); in orange, CD14⁺CD16^low/- and, superimposed, the CD14⁺CD16⁺ population in blue (B). Combining the plots (C) showed the difference of size and granularity of these cells. Data shown are for one representative animal out of six. (D, E) Box and whiskers plots for the arithmetic mean SSC-H (D) and FSC-H (E) for each myeloid subset (n = 6) are presented as the mean values ± SE, showing the difference in size and granularity of the populations. ** denotes significant difference (p < 0.001).