Table 1.
Antibodies list
| Antigen | Antibody nomenclature | Isotype | Conjugate | Source |
|---|---|---|---|---|
| CD1b | CC14 | IgG1 | Nil | IAH, AbD Serotec |
| CD80 | IL-A159 | IgG1 | Nil | ILRI, AbD Serotec |
| CD86 | IL-A190 | IgG1 | Nil | ILRI, AbD Serotec |
| CD40 | IL-A156 | IgG1 | Nil | ILRI, AbD Serotec |
| MHCII-DR | CC108 | IgG1 | Nil | IAH, AbD Serotec |
| CD11b | CC94 | IgG1 | Nil | IAH |
| CD11c | NAM4 | IgG1 | Nil | [44] |
| CD2 | CC42 | IgG1 | Nil | IAH, AbD Serotec |
| CD3 | MM1A | IgG1 | Nil | VMRD, Inc., Pullman, WA |
| CD4 | CC30 | IgG2a | R:PE | IAH, AbD Serotec |
| CD8α | CC63 | IgG2a | R:PE | IAH, AbD Serotec |
| CD8β | CC58 | IgG1 | R:PE | IAH, AbD Serotec |
| CD21 | CC21 | IgG1 | Nil | IAH, AbD Serotec |
| NKp46 | GR13.1(EC1.1) | IgG1 | Nil | Timothy Connelly (UoE), AbD Serotec |
| CD26 | CC69 | IgG1 | Nil | IAH |
| CD172a | CC149 | IgG1 | Nil | IAH |
| CD206 | 3.29B1.10 | IgG1 | R:PE | Beckman Coulter |
| TLR2 | HuCaL AbD 16476.1 | Nil | AbD Serotec | |
| CD163 | EDHu-1 | IgG1 | Nil | AbD Serotec |
| CD16 | KD1 | IgG2a | FITC | AbD Serotec |
| CD14 | TUK4 | IgG2a | Alexa Fluor 647 | AbD Serotec |
| IgG1 isotype control | AV20 | IgG1 | Nil | IAH, [35] |
| IgG2a isotype control | AV29 | IgG2a | Nil | IAH, [35] |
Monoclonal antibodies used for flow cytometric analysis. IAH, Institute for Animal Health; ILRI, International Livestock Research Institute; UoE, University of Edinburgh.