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. 2015 Sep 24;15:17. doi: 10.1186/s12907-015-0017-1

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© Bradley et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Correlation of gene expression of 91 genes from 10 snap-frozen TCGA samples measured with Affymetrix U133 microarray (X-axis) and, in the current study, with TaqMan qPCR (Y-axis). The 91 probes from the 10 samples were each normalized to the average of three housekeeping genes (GUSB, GAPDH, and HPRT1). a The scatterplot shows that gene-to-gene expression has similar ranges across both technologies when normalized to the same three-gene average (r = 0.60). b Lowess smoothing curves. Red dots signify Ct values >34 which are not included in final index measurements