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Effect of substrate mixtures on the cleavage of a specific peptide substrate (SA, SB, SE, or SF) by its corresponding enzyme (A) BoNT/A; (B) BoNT/B; (C) BoNT/E; or (D) BoNT/F. (E) The hydrolysis of SA by BoNT/A toxin in the presence of SE at various concentrations.