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. Author manuscript; available in PMC: 2015 Nov 4.
Published in final edited form as: Anal Chem. 2014 Oct 20;86(21):10847–10854. doi: 10.1021/ac502948v

Table 3.

Measurement of the toxin activities of two BoNT toxins expressed in the cell culture of the bivalent BoNT strains (Ba) under different growing temperatures by the two-stage method.*

Culture Temp. (°C) BoNT/A BoNT/B
Rx Time (hr) Measured Amt (mLD50) Amt30°C/Amt37°C Rx Time (hr) Measured Amt (mLD50) Amt30°C/Amt37°C
30 4.0 0.09 0.8 0.5 243.0 0.9
37 4.0 0.11 0.5 257.8
*

The cell culture samples were diluted by 100 fold and 5μl of the resulting solutions were subjected to toxin enrichment and two-stage quantitative Endopep-MS assay.