Table 3.

Hydrolysis of the truncated SNAP-25 peptides by BoNT/A1 and /A5.^*

Peptide	RateA1 (μM/min)	RateA5 (μM/min)	RateA5/RateA1
171–206	0.008	0.002	0.2
161–206	0.007	0.003	0.4
156–206	0.020	0.014	0.7
151–206	0.018	0.097	5.5
146–206	0.016	0.094	5.7
155–206	0.024	0.019	0.8
154–206	0.015	0.018	1.2
153–206	0.019	0.029	1.5
152–206	0.024	0.053	2.2
153–206/G155K	0.069	0.060	0.9
153–206/S154A	0.026	0.041	1.6
153–206/S154hS	0.016	0.023	1.4
153–206/S154K	0.065	0.057	0.9
153–206/S154R	0.060	0.058	1.0
153–206/V153A	0.029	0.033	1.1
153–206/V153K	0.076	0.053	0.7
153–206/V153D	0.013	0.051	4.0
153–206/V153E	0.016	0.050	3.2
153–206/E151A	0.036	0.082	2.3

^*Cleavage reaction condition: 1nM toxin, 20uM peptide substrate, 37°C for 10 – 30 min.