Fig. 1.

Phenotype and trilineage potential of CD271-MSCs. a Potential to generate CFU-Fs. Positively selected CD271⁺ BM-MNCs as well as non-selected BM-MNCs were incubated for 14 days. Next, generated CFU-Fs were stained with Giemsa (blue spots on the bottom of tissue culture flasks) and then counted. Positively selected CD271⁺ BM-MNCs gave rise to a significantly higher number of CFU-Fs than non-selected BM-MNCs (P < 0.003). Results are persented as mean value ± standard error mean (n = 4). b Phenotype of CD271-MSCs was determined at the end of P2 and P4. Both types of MSCs studied revealed the same phenotype which is typcial for MSCs (negative for hematopoeitic markers and HLA-DR and positive for CD73, CD90, CD105 and HLA-Class I antigens). c Trilineage differentiation potential of both types of MSCs was evaluated by their culture in tissue-specific media for adipocytes, osteoblasts and chondrocytes. Confirmation of trilineage differentiation of MSCs was performed by staining with Oil Red O staining solution for adipocytes, SIGMA FAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets for osteoblasts and alcian blue solution for chondrocytes