Fig. 2.

Effect of pH on InpA activity towards Z-Arg-Arg-AMC (a) and azoalbumin (b). Protease activity was assayed at 37 °C in PBS, pH 7.4, containing 3 mM DTT. InpA concentration was 50 μg ml⁻¹. Azoalbumin concentration was 3 % (w/v). Enzyme activity is depicted as absorbance of azo dye (A₄₄₀) released from the protein. See text for details