Fig. 4.

Chemical reduction increases the susceptibility of albumin to InpA. Albumin (16 μM) was incubated with InpA (100 μg ml⁻¹) at different pH at 37 °C under non-reducing (a panels) and reducing conditions (3 mM DTT) (b panels). Gel lanes had a nominal loading of 20 μg albumin, and are stained with CBB. Samples were prepared and gels run as described for Fig. 3