Table 1. Total pyrosequencing dataset statistics, including statistics for potential mRNA transcripts data subsets.
| Full dataset stats | Statistics for subsets of potential mRNA transcripts | |||||
|---|---|---|---|---|---|---|
| Sample/time of day | Total no. of reads | Avg. read length (bp) | No. of potential mRNA reads (% of total) | No. of potential mRNA reads with no significant BLASTX alignments (%) | No. of potential mRNA reads functionally annotated into MG-RAST subsystems (%) | % potential mRNA reads taxonomically unclassifieda |
| 22:15 | 297,646 | 427 | 34,835 (11.7%) | 15,935 (45.7%) | 7,730 (22.2%) | 10.2% |
| 07:30 | 301,933 | 412 | 53,381 (17.7%) | 26,079 (48.9%) | 14,212 (26.6%) | 10.4% |
| 12:15 | 324,282 | 402 | 35,643 (11.0%) | 15,938 (44.7%) | 8,945 (25.1%) | 13.4% |
| 12:15 (total RNA control) | 198,212 | 344 | 10,035 (5.1%) | – | – | – |
Notes.
a
Unassigned at the phylum-level and includes the sum of all potential mRNA reads assigned by MEGAN 4.0 as Unassigned Bacteria, Unassigned Archaea, and Unassigned Cellular Organisms.