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. 2015 Jul 6;146(2):251–263. doi: 10.1111/imm.12497

Figure 2.

Figure 2

p38 activation modulates interleukin-17 (IL-17) release and RORC and IL-17 expression in vitro: (a) IL-17 levels in the supernatants from three distinct differentiation assays were assessed by ELISA. (b) FACS-sorted CD4+ CD45RA+ CD27+ naive T cells were stimulated with anti-CD3 and anti-CD28 in the presence or absence of the p38-inhibitor BIRB796. The cocktail of cytokines [transforming growth factor-β (TGF-β), interleukin-1 β (IL-1β), IL-6 and IL-23] was added to the culture where specified. After 5 days the cells were harvested and mRNA levels of the transcription factor RORγt (left panel) and of the cytokine IL-17A (right panel) were measured by quantitative RT-PCR. *P < 0·05, **P < 0·01; ***P < 0·001.